Chloroplast Genetic Engineering
نویسندگان
چکیده
منابع مشابه
Genetic engineering of the chloroplast: novel tools and new applications.
The plastid genome represents an attractive target of genetic engineering in crop plants. Plastid transgenes often give high expression levels, can be stacked in operons and are largely excluded from pollen transmission. Recent research has greatly expanded our toolbox for plastid genome engineering and many new proof-of-principle applications have highlighted the enormous potential of the tran...
متن کاملPhytoremediation of organomercurial compounds via chloroplast genetic engineering.
Mercury (Hg), especially in organic form, is a highly toxic pollutant affecting plants, animals, and man. In plants, the primary target of Hg damage is the chloroplast; Hg inhibits electron transport and photosynthesis. In the present study, chloroplast genetic engineering is used for the first time to our knowledge to enhance the capacity of plants for phytoremediation. This was achieved by in...
متن کاملAdvances in chloroplast engineering.
The chloroplast is a pivotal organelle in plant cells and eukaryotic algae to carry out photosynthesis, which provides the primary source of the world's food. The expression of foreign genes in chloroplasts offers several advantages over their expression in the nucleus: high-level expression, transgene stacking in operons and a lack of epigenetic interference allowing stable transgene expressio...
متن کاملGenetic Analysis of Chloroplast Translation
This project concerned two nuclear genes in maize, crp1 and atp1, whose function is required for the translation of one or several chloroplast mRNAs. Mutations in these genes cause a reduction in the number of ribosomes bound to the regulated mRNAs, implying a defect in translation initiation or early elongation. The proposed experiments were designed to elucidate how their gene products activa...
متن کاملCodon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii
There is a growing interest in the use of microalgae as low-cost hosts for the synthesis of recombinant products such as therapeutic proteins and bioactive metabolites. In particular, the chloroplast, with its small, genetically tractable genome (plastome) and elaborate metabolism, represents an attractive platform for genetic engineering. In Chlamydomonas reinhardtii, none of the 69 protein-co...
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ژورنال
عنوان ژورنال: Biotechnology Journal
سال: 2006
ISSN: 1860-6768,1860-7314
DOI: 10.1002/biot.200690011